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Loading Buffer Product List

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Loading Buffer

Loading Buffer

Contains SDS! The dyes are bromophenol blue and xylene cyanol.

"Loading Buffer" is a reagent added when preparing nucleic acid samples for electrophoresis. It is used by adding 1/5 to 1/10 of the sample volume. It contains two types of dyes (Bromophenol Blue and Xylene Cyanol) and Glycerol. Since this product contains SDS, it may affect the electrophoresis patterns of DNA and bound proteins (such as Taq MutS). On the other hand, if you want to terminate an enzyme reaction or if the sample solution contains a large amount of protein, having SDS included will result in less impact on the electrophoretic mobility. 【Features】 ■ Added when preparing nucleic acid samples for electrophoresis ■ Used by adding 1/5 to 1/10 of the sample volume ■ Contains two types of dyes and Glycerol ■ Affects the electrophoresis patterns of DNA and bound proteins *For more details, please refer to the related links or feel free to contact us.

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6 X Loading Buffer Double Dye

6 X Loading Buffer Double Dye

The dye is Orange G and Xylene Cyanol FF (XC)! It is possible to take UV photos without any dye shadows in the central part of the gel.

"6 X Loading Buffer Double Dye" is a reagent added when preparing nucleic acid samples for electrophoresis. By not using BPB, it is possible to take UV photographs without any dye shadows in the center of the gel. As an example of use, add 2μl of this product to a 10μl DNA sample solution, mix by pipetting several times, and then gently inject (apply) 10μl of the mixture into the wells of an agarose gel to perform electrophoresis. 【Features】 - Added when preparing nucleic acid samples for electrophoresis - Used at 1/6 the amount relative to the sample volume - BPB-free - Less prone to band smiling compared to glycerol *For more details, please refer to the related links or feel free to contact us.

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6 X Loading Buffer Triple Dye

6 X Loading Buffer Triple Dye

Compared to glycerol, band smiling is less likely to occur! Introducing the reagents we handle.

"6 X Loading Buffer Triple Dye" is a reagent added when preparing nucleic acid samples for electrophoresis. It is used by adding 1/6 of the amount relative to the sample volume. The dyes used include Orange G, which allows for the visualization of the nearly leading edge of the electrophoresed sample, Bromophenol Blue (BPB), and Xylene Cyanol FF (XC), which helps confirm the mobility of high molecular weight substances. Ficoll PM400 is used as a density additive, which reduces the occurrence of band smearing compared to glycerol. 【Features】 - Added when preparing nucleic acid samples for electrophoresis - Used by adding 1/6 of the amount relative to the sample volume - Ficoll PM400 is used as a density additive - Reduced band smearing compared to glycerol *For more details, please refer to the related links or feel free to contact us.

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6 x Loading Buffer Orange G

6 x Loading Buffer Orange G

Add 1/6 of the sample volume for use! Orange G allows you to see almost the tip of the migrating substance.

"6 x Loading Buffer Orange G" is a reagent added when preparing nucleic acid samples for electrophoresis. Use 1/6 of the amount relative to the sample volume. Ficoll PM400 is used as the density additive, which reduces the occurrence of band smiling compared to glycerol. Additionally, by not using BPB, it is possible to take UV photographs of the gel's central area without any dye shadow during photography. 【Features】 ■ Added when preparing nucleic acid samples for electrophoresis ■ BPB is not used ■ Almost the leading edge of the migrating substance can be observed ■ Ficoll PM400 is used as the density additive *For more details, please refer to the PDF document or feel free to contact us.

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2 X DGGE Loading Buffer

2 X DGGE Loading Buffer

The packaging unit is 10 ml! Introducing the reagents for denaturing gradient gel electrophoresis that we handle.

"2 X DGGE Loading Buffer" is a product for DGGE (Denaturing Gradient Gel Electrophoresis). Use half the amount relative to the sample volume. It contains Bromophenol Blue and Glycerol. As an example of use, add an equal amount of this product to the DNA sample solution, mix by pipetting several times, and then gently apply to the wells of the denaturing gradient gel for electrophoresis. 【Features】 ■ For DGGE ■ Use half the amount relative to the sample volume ■ Contains Bromophenol Blue and Glycerol *For more details, please refer to the related links or feel free to contact us.

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